Open AccessGlobal circular RNA expression profile of human gastric cancer and its clinical significance
Author(s) -
Shao Yongfu,
Li Jinyun,
Lu Rongdan,
Li Tianwen,
Yang Yunben,
Xiao Bingxiu,
Guo Junming
Publication year - 2017
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1055
Subject(s) - rna , microbiology and biotechnology , cancer , circular rna , carcinoembryonic antigen , downregulation and upregulation , biology , gene , biochemistry , genetics
Circular RNA s (circ RNA s) are a new class of noncoding RNA s. However, the expression profile and clinical significance of circ RNA s in human gastric cancer is unclear. The global circ RNA expression profile in human gastric cancer was measured by circ RNA microarray. Hsa_circ_0014717, one of the most downregulated circ RNA s in microarray, was selected as a targeted circ RNA to explore its levels in gastric tissues and gastric juice. Freeze‐thaw experiment and incubation experiment confirmed the stability of gastric juice circ RNA s. A total of 308 circ RNA s, including 107 (34.74%) upregulated and 201 (65.26%) downregulated circ RNA s, were found significantly aberrantly expressed in gastric cancer tissues. The top ten upregulated in gastric cancer tissues were hsa_circ_0035445, hsa_circ_0003789, hsa_circ_0063809, hsa_circ_0074362, hsa_circ_0006282, hsa_circ_0011107, hsa_circ_0084606, hsa_circ_0005556, hsa_circ_0050547, and hsa_circ_0006470, while the top ten downregulated ones were hsa_circ_0007099, hsa_circ_0001897, hsa_circ_0007707, hsa_circ_0008832, hsa_circ_0001546, hsa_circ_0002089, hsa_circ_0004680, hsa_circ_0000154, hsa_circ_0004458, and hsa_circ_0008394. The hot‐point chromosomes were chr1, chr2, chr3, chr9, and chr17. Hsa_circ_0014717 was significantly downregulated in 77.2% (74/96) gastric cancer tissues. Its levels in gastric cancer tissues were related to tumor stage ( P = 0.037), distal metastasis ( P = 0.048), tissue carcinoembryonic antigen ( P = 0.001), and carbohydrate antigen 19‐9 expression ( P = 0.021). More importantly, hsa_circ_0014717 can stably exist in human gastric juice; and its nature meets the requirements of clinical detection. Our study uncovered the circ RNA expression profile in human gastric cancer. Moreover, some circ RNA s can stably exist in human body fluid, and has the potential to be used as novel biomarkers for the screening of high‐risk gastric cancer patients.