Open AccessRetracted: Effects of miR‐145‐5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI 3K/ AKT pathways
Author(s) -
Liu Sha,
Gao Guozhen,
Yan Dexiong,
Chen Xiangjun,
Yao Xingwei,
Guo Shuzhong,
Li Guirong,
Zhao Yu
Publication year - 2017
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1030
Subject(s) - neuroblastoma ras viral oncogene homolog , apoptosis , mapk/erk pathway , cancer research , cell growth , melanoma , flow cytometry , cell migration , pi3k/akt/mtor pathway , viability assay , chemistry , biology , cell , microbiology and biotechnology , signal transduction , cancer , kras , biochemistry , genetics , colorectal cancer
Abstract We aimed to detect the effects of miR‐145‐5p on the cell proliferation, apoptosis, migration, and invasion in NRAS ‐mutant, BRAF ‐mutant, and wild‐type melanoma cells, in order to figure out the potential mechanisms and provide a novel therapeutic target of melanoma. RT ‐ qPCR and western blot were used to detect the expression of miR‐145‐5p and NRAS in melanoma tumor tissues and cells, respectively. Luciferase assay was performed to determine whether miR‐145‐5p directly targeted NRAS . After transfecting miR‐145‐5p mimics, miR‐145‐5p inhibitors, NRAS cDNA and NRAS siRNA into CHL‐1, VMM917 and SK‐mel‐28 cells, functional assays were used to detect the proliferation, apoptosis, invasion and migration, including MTT, flow cytometry, Transwell and wound healing assays. In addition, xenograft models in nude mice were also conducted to verify the role of miR‐145‐5p in vivo. MiR‐145‐5p was able to suppress proliferation, invasion, and migration of VMM 917 and CHL ‐1 cells and induce apoptosis by inhibiting MAPK and PI 3K/ AKT pathways. However, aberrant expression of miR‐145‐5p and NRAS has little impact on the viability and metastasis of BRAF ‐mutant melanoma. The higher expression of miR‐145‐5p in xenograft models repressed the VMM 917‐induced and CHL ‐1‐induced tumor growth observably and has little effect on SK ‐mel‐28‐induced tumor growth which was consistent with the results in vitro. Through targeting NRAS , miR‐145‐5p could suppress cell proliferation, invasion, and migration and induce apoptosis of CHL ‐1 and VMM 917 melanoma cells by inhibiting MAPK and PI 3K/ AKT pathways.