Open AccessDRP5 is involved in cancer cell growth and predicts poor prognosis in human osteosarcoma
Author(s) -
Wang Lin,
Liu Weihai,
Tang Hengtao,
Xie Xianbiao,
Zou Changye,
Wang Yongqian,
Gao Zhenhua,
Yin Junqiang
Publication year - 2017
Publication title -
cancer medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.403
H-Index - 53
ISSN - 2045-7634
DOI - 10.1002/cam4.1009
Subject(s) - osteosarcoma , gene knockdown , cancer research , gene silencing , tissue microarray , downregulation and upregulation , cell growth , immunohistochemistry , biology , cancer , cell culture , medicine , pathology , gene , genetics
Osteosarcoma is an extremely aggressive primary malignant bone tumor of childhood. Collapsin response mediator proteins ( CRMP s), which are highly expressed in the developing nervous system, were recently shown to be associated with cancer development. However, the relationship between DRP 5 ( CRMP 5) and osteosarcoma has not been evaluated. In this study, we investigated the role of DRP 5 in the regulation of osteosarcoma growth. DRP 5 mRNA and protein levels were significantly upregulated in human osteosarcoma cell lines and associated with increased migration and invasion. Genetic knockdown of DRP 5 markedly suppressed the expression of matrix metalloproteinase ( MMP )‐2 and MMP ‐9. DRP 5 silencing significantly inhibited osteosarcoma cell growth in vitro and in a xenograft mouse model in vivo. Microarray immunohistochemical analysis of osteosarcoma specimens and Kaplan–Meier analysis showed that patients with high DRP 5 protein expression had shorter overall survival than those with low DRP 5 levels. Taken together, these results suggest that DRP 5 plays a critical role in the regulation of osteosarcoma and could be a potential therapeutic target and prognostic factor in osteosarcoma.