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Inhibitory effects of a manganese superoxide dismutase isolated from garlic ( Allium sativum L.) on in vitro tumoral cell growth
Author(s) -
Sfaxi Imen Hadji,
Ferraro Daniela,
Fasano Elena,
Pani Giovambattista,
Limam Ferid,
Marzouki M. Nejib
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.94
Subject(s) - superoxide dismutase , reactive oxygen species , superoxide , biochemistry , allium sativum , chemistry , viability assay , dismutase , cell growth , microbiology and biotechnology , enzyme , biology , cell , botany
Abstract Reactive oxygen species are implicated in cancer development and antioxidants in general and superoxide dismutases and superoxide dismutase mimetic in particular, and they inhibit malignant transformation. We examinate the effects of an isolated manganese superoxide dismutase from a medicinal plant Allium sativum . The protein was prepared by a serial of chromatographic techniques: gel filtration and diethylaminoethyl ions exchanger. The enzyme has a specific activity equal to 55 U/mg. Two tumoral cell lines, porcine endothelial cells and mouse melanoma cells were exposed to garlic superoxide dismutase. The exogenous manganese superoxide dismutase is able to modify the intracellular level of reactive oxygen species by eliminating superoxide anion and producing hydrogen peroxide. The cell viability of the two lines was not significantly affected but the cell multiplication was arrested. This effect obtained in the presence of manganese superoxide dismutase correlates with the activation and modulation of phospho‐extracellular signal‐regulated kinases proteins, implicated in the control of several biological processes including cell proliferation. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009