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Formation of cyclodextrins with cyclodextrin glucosyltransferase stimulated with polarized light
Author(s) -
Fiedorowicz Maciej,
Khachatryan Gohar,
Konieczna–Molenda Anna,
Tomasik Piotr
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.90
Subject(s) - glucosyltransferase , cyclodextrin , yield (engineering) , enzyme , chemistry , glucosyltransferases , white light , enzyme assay , photochemistry , biochemistry , materials science , optoelectronics , metallurgy
After illumination with white, linearly polarized light (WLPL), cyclodextrin glycosyltransferase produced mixture of α‐, β‐, and γ‐cyclodextrins (CD) with higher overall yield than did that enzyme when nonilluminated. The illumination also influenced the ratio of those CD and that effect depended on concentration of enzyme and illumination time. At a high enzyme concentration (0.64 U/cm 3 ), regardless the illumination time, formation of β‐CD predominated. The highest yield of β‐CD was afforded after 1 h illumination and 2 h illumination led to a significant increase in the yield of γ‐CD. Three‐month storage of enzyme illuminated with WLPL did not reduce its enhanced activity. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009