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Characterization of gene localization and accessibility in DHFR‐amplified CHO cells
Author(s) -
Jiang Zhou,
Sharfstein Susan T.
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.82
Subject(s) - chinese hamster ovary cell , biology , dihydrofolate reductase , epigenetics , gene , transgene , genetics , transcription (linguistics) , transcriptional regulation , microbiology and biotechnology , gene duplication , gene expression , cell culture , linguistics , philosophy
Efficient transcription is critical for high yields of recombinant proteins by mammalian cells. We previously reported that dihydrofolate reductase (DHFR)‐mediated gene amplification can augment transcriptional rates as well as increasing gene copy numbers in Chinese hamster ovary (CHO) cells.1 In an attempt to elucidate the mechanisms involved, we have employed several approaches to identify the epigenetic differences between cell clones with varying transcriptional rates. Transgene placement and accessibility varies between unrelated parental cell clones with differential transcriptional rates. However, we did not observe any apparent epigenetic differences between parental clones and their amplified progeny, indicating undiscovered regulatory mechanisms are responsible for the augmentation of transcriptional rates upon DHFR‐mediated amplification. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009