Premium
Expression system for recombinant human growth hormone production from Bacillus subtilis
Author(s) -
Özdamar Tunçer H.,
Şentürk Birgül,
Yilmaz Özge Deniz,
Çalık Güzide,
Çelik Eda,
Çalık Pınar
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.81
Subject(s) - bacillus subtilis , serine protease , recombinant dna , signal peptide , biology , biochemistry , bacillus licheniformis , serine , protease , microbiology and biotechnology , complementary dna , heterologous expression , gene , chemistry , enzyme , bacteria , genetics
We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis . A hybrid‐gene of two DNA fragments, i.e., signal ( pre‐ ) DNA sequence of B. licheniformis serine alkaline protease gene ( sub C) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg ‐promoter in B. subtilis . Recombinant‐hGH (rhGH) produced by B. subtilis carrying pMK4:: pre(subC) :: hGH was secreted. N‐terminal sequence and mass spectrometry analyses of rhGH confirm the mature hGH sequence, and indicate that the signal peptide was properly processed by B. subtilis signal‐peptidase. The highest rhGH concentration was obtained at t = 32 h as C rhGH = 70 mg L −1 with a product yield on substrate Y rhGH/S = 9 g kg −1 , in a glucose based defined medium. Fermentation characteristics and influence of hGH gene on the rhGH production were investigated by comparing B. subtilis carrying pMK4:: pre(subC) :: hGH with that of carrying merely pMK4. Excreted organic‐acid concentrations were higher by B. subtilis carrying pMK4:: pre(subC) :: hGH , whereas excreted amino‐acid concentrations were higher by B. subtilis carrying pMK4. The approach developed is expected to be applicable to the design of expression systems for heterologous protein production from Bacillus species. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009