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Development of a mathematical model for evaluating the dynamics of normal and apoptotic Chinese hamster ovary cells
Author(s) -
Naderi Saeideh,
Meshram Mukesh,
Wei Catherine,
McConkey Brendan,
Ingalls Brian,
Budman Hector,
Scharer Jeno
Publication year - 2011
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.647
Subject(s) - chinese hamster ovary cell , monoclonal antibody , biological system , cell culture , flux (metallurgy) , metabolic flux analysis , ovary , hamster , growth model , biology , model system , cell growth , microbiology and biotechnology , system dynamics , computational biology , metabolism , chemistry , biochemistry , mathematics , antibody , immunology , genetics , organic chemistry , mathematical economics , ecology
A metabolic flux based methodology was developed for modeling the metabolism of a Chinese hamster ovary cell line. The elimination of insignificant fluxes resulted in a simplified metabolic network which was the basis for modeling the significant metabolites. Employing kinetic rate expressions for growing and non‐growing subpopulations, a logistic model was developed for cell growth and dynamic models were formulated to describe culture composition and monoclonal antibody (MAb) secretion. The model was validated for a range of nutrient concentrations. Good agreement was obtained between model predictions and experimental data. The ultimate goal of this study is to establish a comprehensive dynamic model which may be used for model‐based optimization of the cell culture for MAb production in both batch and fed‐batch systems. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011