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Effects of extracellular matrices derived from different cell sources on chondrocyte functions
Author(s) -
Hoshiba Takashi,
Lu Hongxu,
Yamada Tomoe,
Kawazoe Naoki,
Tateishi Tetsuya,
Chen Guoping
Publication year - 2011
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.592
Subject(s) - chondrocyte , microbiology and biotechnology , extracellular matrix , mesenchymal stem cell , tissue engineering , chemistry , cell adhesion , cellular differentiation , cell , fibroblast , cell growth , cartilage , biology , in vitro , anatomy , biochemistry , genetics , gene
Cell‐derived extracellular matrices (ECMs) are a key factor in regulating cell functions in tissue engineering and regenerative medicine. The fact that cells are surrounded by their specific ECM in vivo elicits the need to elucidate the effects of ECM derived from different cell sources on cell functions. Here, three types of ECM were prepared by decellularizing cultured chondrocytes, fibroblasts, and mesenchymal stem cells (MSC) and used for chondrocyte culture to compare their effects on chondrocyte adhesion, proliferation, and differentiation. Chondrocyte adhesion to the chondrocyte‐derived ECM was greater than those to the fibroblast‐ and MSC‐derived ECM. Chondrocyte proliferation on the chondrocyte‐derived ECM was lower than those on the fibroblast‐ and MSC‐derived ECM. The ECM showed no evident effect on chondrocyte differentiation. The effects of ECM on cell functions depended on the cell source used to prepare the ECM. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011