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Biocatalytic generation of Mn(III)‐chelate as a chemical oxidant of different environmental contaminants
Author(s) -
TaboadaPuig Roberto,
LúChau Thelmo,
Eibes Gemma,
Moreira María Teresa,
Feijoo Gumersindo,
Lema Juan Manuel
Publication year - 2011
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.585
Subject(s) - chemistry , malonate , manganese peroxidase , oxidizing agent , anthracene , effluent , organic chemistry , aromatic hydrocarbon , hydrocarbon , nuclear chemistry , enzyme , peroxidase , waste management , engineering
The objective of this study was to investigate the enzymatic generation of the Mn 3+ ‐malonate complex and its application to the process of oxidizing several organic compounds. The experimental set‐up consisted of an enzymatic reactor coupled to an ultrafiltration membrane, providing continuous generation of Mn 3+ ‐malonate from a reaction medium containing versatile peroxidase (an enzyme produced by Bjerkandera adusta strain BOS55), H 2 O 2 , MnSO 4, and malonate. The effluent of the enzymatic reactor was introduced into a batch‐stirred reactor to oxidize three different classes of compounds: an azo dye (Orange II), three natural and synthetic estrogens, and a polycyclic aromatic hydrocarbon (anthracene). The enzymatic reactor provided the Mn 3+ complex under steady‐state conditions, and this oxidative species was able to transform the three classes of xenobiotics considerably (90–99%) with negligible loss of activity. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011