Premium
Metabolic Pathway Rates and Fluorescence Measurements During Bioconversions by Non‐Growing Immobilized Clostridium Acetobutylicum
Author(s) -
Reardon Kenneth F.,
Bailey James E.
Publication year - 1989
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420050404
Subject(s) - clostridium acetobutylicum , lysis , biochemistry , fluorometer , mutant , metabolic pathway , strain (injury) , chemistry , clostridium , spore , biology , metabolism , fluorescence , ethanol , microbiology and biotechnology , bacteria , butanol , gene , physics , genetics , anatomy , quantum mechanics
Metabolic and physiological aspects of non‐growing calcium alginate‐immobilized Clostridium acetobutylicum were investigated in a continuous‐flow reactor system. Changes in metabolic reaction rates and product selectivity were analyzed via metabolic pathway rate analysis, and variations in intracellular NAD(P)H‐dependent fluorescence were monitored using an on‐line fluorometer. In addition, the effects of sporulation were studied by comparing experiments utilizing either a sporulating strain (ATCC 824) or a sporulation mutant (ATCC 39236). In both cases, biocatalytic activity was lost through an initial period of cell lysis; afterwards, the sporulation mutant retained overall activity for a longer time because of the much lower degree of sporulation in this strain. The analysis of metabolic pathway rates in both experiments showed that early formation of solvent products was replaced by acid product formation, that the specific rates of all metabolic reactions were likely enhanced by the release of cytoplasmic compounds from the lysed cells (to a greater extent for the ATCC 824 cells), and that the patterns of specific activity loss were different between the two strains.