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Growth Kinetics of Strawberry Cell Suspension Cultures in Shake Flask, Airlift, Stirred‐Jar, and Roller Bottle Bioreactors
Author(s) -
Hong Y. C.,
Labuza T. P.,
Harlander S. K.
Publication year - 1989
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420050403
Subject(s) - bioreactor , airlift , bottle , baffle , suspension (topology) , suspension culture , fragaria , chromatography , oxygen , chemistry , botany , materials science , biology , chemical engineering , cell culture , composite material , mathematics , genetics , organic chemistry , homotopy , pure mathematics , engineering
Growth kinetics of strawberry cell suspension cultures (Fragaria × ananassa cv. Brighton) in shake flask, airlift, stirred‐jar, and roller bottle bioreactors were investigated. The specific oxygen consumption rates of strawberry suspension cultures in shake flasks ranged from 0.10 to 0.17 mmol O 2 /gDCW/hr. These values were relatively low compared to published values of specific oxygen consumption rates in other plant tissue culture systems. The volumetric oxygen‐transfer coefficients in bioreactor systems were determined and found to be influenced by both agitation speed and air flow rate. The specific growth rates of strawberry cell suspension cultures were higher in the roller bottle with baffles (0.15 day −1 ) than any other system studied. It was difficult to grow cell suspension cultures in the stirred‐jar bioreactor. The roller bottle with baffles shows the best potential for scale‐up.