Recovery of an Extracellular Alkaline Protease From Whole Fermentation Broth Using Reversed Micelles | Zendy

Rahaman R. S. | Zendy; Chee J. Y. | Zendy; Cabral J. M. S. | Zendy; Hatton T. A. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Recovery of an Extracellular Alkaline Protease From Whole Fermentation Broth Using Reversed Micelles

Author(s) -

Rahaman R. S.,

Chee J. Y.,

Cabral J. M. S.,

Hatton T. A.

Publication year - 1988

Publication title -

biotechnology progress

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.572

H-Index - 129

eISSN - 1520-6033

pISSN - 8756-7938

DOI - 10.1002/btpr.5420040405

Subject(s) - chromatography , chemistry , fermentation , micelle , alkaline protease , extraction (chemistry) , protease , extracellular , solvent , enzyme , aqueous solution , biochemistry , organic chemistry

The liquid‐liquid extraction of an extracellular alkaline protease from its fermentation broth was carried out, using a reversed micellar organic solvent. Optimum process parameters were determined for extraction using a 250 mM AOT in isooctane micellar solution. With unequal phase volume ratios, purification factors as high as 6 have been obtained from completely untreated broth. Yields up to 56% were achieved from the same broth using a three‐stage cascade.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research