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Recovery of an Extracellular Alkaline Protease From Whole Fermentation Broth Using Reversed Micelles
Author(s) -
Rahaman R. S.,
Chee J. Y.,
Cabral J. M. S.,
Hatton T. A.
Publication year - 1988
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420040405
Subject(s) - chromatography , chemistry , fermentation , micelle , alkaline protease , extraction (chemistry) , protease , extracellular , solvent , enzyme , aqueous solution , biochemistry , organic chemistry
The liquid‐liquid extraction of an extracellular alkaline protease from its fermentation broth was carried out, using a reversed micellar organic solvent. Optimum process parameters were determined for extraction using a 250 mM AOT in isooctane micellar solution. With unequal phase volume ratios, purification factors as high as 6 have been obtained from completely untreated broth. Yields up to 56% were achieved from the same broth using a three‐stage cascade.