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Formulation and Characterization of a Multiple Emulsion for Use as a Red Blood Cell Substitute
Author(s) -
Borwanker C. M.,
Pfeiffer S. B.,
Zheng S.,
Beissinger R. L.,
Wasan D. T.,
Sehgal L. R.,
Rosen A. L.
Publication year - 1988
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420040404
Subject(s) - emulsion , hemoglobin , yield (engineering) , chemistry , blood substitute , chromatography , chemical engineering , oxygen , materials science , biochemistry , organic chemistry , composite material , engineering
A new approach to the development of an oxygen‐carrying blood substitute is presented. The development of a viable blood substitute in the form of a multiple emulsion encapsulating hemoglobin solution was explored. Results indicated that the formulation ingredients and procedure affect the properties of the multiple emulsion. In order to evaluate the different formulations a quantitative method of assessing the encapsulation efficiency (yield) of the multiple emulsions was developed. The yield test measures the amount of hemoglobin released into the external phase. A model mutiple emulsion was formulated and characterized by yield, drop size distribution, oxygen‐carrying capacity, oxyhemoglobin dissociation, short‐term storage stability, and viscosity. The yield obtained for the model formulation was excellent (greater than 90%) giving high oxygen‐carrying capacity. The high yield was maintained over a four day storage period at 4d̀C.