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Protein Coated Adsorbents for use in Potential Barrier Chromatography: Fouling Chromatography
Author(s) -
Lesins V.,
Ruckenstein E.
Publication year - 1988
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420040104
Subject(s) - adsorption , fouling , chromatography , chemistry , protein adsorption , hydrophobic effect , hydrophilic interaction chromatography , protein purification , chemical engineering , membrane , organic chemistry , high performance liquid chromatography , biochemistry , engineering
Fouling is employed as a method of obtaining adsorbents suitable for use in Potential Barrier Chromatography. A relatively inexpensive hydrophobic adsorbent, which is initially unsuitable for protein separations, is intentionally fouled with protein. The packing slowly evolves from an adsorbent which binds proteins strongly, due to strong hydrophobic interactions, to a protein coated adsorbent in which weaker hydrophobic interactions are opposed by enhanced double layer repulsion. As a result, an adsorbent which is suitable for protein separations evolves. Details regarding the fouling process, the chromatographic separation of two model proteins and the physical forces involved are outlined.