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Polymer Dosage Considerations in Polyelectrolyte Precipitation of Protein
Author(s) -
Clark K. M.,
Glatz C. E.
Publication year - 1987
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420030408
Subject(s) - carboxymethyl cellulose , polyelectrolyte , polymer , precipitation , particle size , chemistry , chromatography , lysozyme , chemical engineering , zeta potential , particle (ecology) , polymer chemistry , materials science , organic chemistry , biochemistry , sodium , biology , nanoparticle , ecology , physics , meteorology , engineering
The properties of aggregates formed by the precipitation of egg white proteins by carboxymethyl cellulose (CMC) were examined. Both the final level and the number of incremental additions of the polyelectrolyte were varied. The protein and lysozyme recoveries, particle zeta potential, and protein composition of the precipitate were found to vary with only the final CMC level. Particle size and size distributions were dependent on both the polymer dosage and manner of addition. Overdosed systems, or systems in which the CMC dosage exceeded that required for optimal total protein removal, produced the smallest particles, with size increasing as the number of incremental polymer additions increased. Optimally dosed systems produced the largest particles.