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Electrophoretic Elution from Monoclonal Antibody Immunoadsorbents: A Theoretical and Experimental Investigation of Controlling Parameters
Author(s) -
Olson William C.,
Yarmush Martin L.
Publication year - 1987
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420030309
Subject(s) - elution , electrophoresis , chemistry , chromatography , monoclonal antibody , saturation (graph theory) , bovine serum albumin , albumin , affinities , analytical chemistry (journal) , antibody , biochemistry , biology , immunology , mathematics , combinatorics
An experimental and theoretical investigation of the mechanism of electrophoretic elution is described. The rate of elution of bovine serum albumin from monoclonal antibody immunoadsorbents was observed to increase with increasing electric field, increasing fractional saturation, and decreasing height of the immunoadsorbent column. Slower elution rates were observed from immunoadsorbents possessing higher affinities and capacities. A reaction‐migration mechanism is proposed in which the packed bed is modeled as a continuous hydrogel and in which the electrical field causes antigen that has spontaneously dissociated to migrate through the column under conditions of bound‐free equilibrium. Good qualitative and quantitative agreement is obtained between experiment and the predictions of a numerical model. A membrane device suitable for large‐scale application of this technique is discussed.