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Metabolic Pathway Rates and Culture Fluorescence in Batch Fermentations of Clostridium Acetobutylicum
Author(s) -
Reardon Kenneth F.,
Scheper ThomasHelmut,
Bailey James E.
Publication year - 1987
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420030307
Subject(s) - clostridium acetobutylicum , acidogenesis , intracellular , clostridium , nad+ kinase , biochemistry , fermentation , chemistry , metabolic pathway , fluorescence , biology , metabolism , enzyme , bacteria , butanol , ethanol , anaerobic digestion , methane , organic chemistry , physics , quantum mechanics , genetics
Stoichiometric relationships in the metabolic pathway of Clostridium acetobutylicum are used to formulate a framework for the analysis of pathway rates. This analytical tool, alone and in conjunction with intracellular NAD(P)H fluorescence measurements, is applied to two batch fermentations, one acidogenic and one solventogenic, to provide information on intracellular activities not readily obtainable by other methods.