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Modeling and Measurement of Yeast Flocculation
Author(s) -
Davis Robert H.,
Hunt Thomas P.
Publication year - 1986
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420020208
Subject(s) - flocculation , settling , sedimentation , yeast , extinction (optical mineralogy) , suspension (topology) , fractal , biological system , fractal dimension , chromatography , chemistry , mineralogy , biology , thermodynamics , physics , environmental science , mathematics , environmental engineering , sediment , biochemistry , paleontology , mathematical analysis , homotopy , pure mathematics
A sedimentation/light extinction technique has been developed to quantitatively measure the state of flocculation of a suspension of yeast cells. This experimental method is based upon sedimentation theory with a modified light extinction theory that accounts for the dependence of the extinction coefficient on the floc size distribution, and the loosely‐branched fractal structure of yeast aggregates. As demonstrated by comparisons with direct microscopic observations, this technique is capable of accurately determining the steady‐state of floc sizes as well as floc settling velocities of a variety of yeast cultures.