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The Relationship Between Cell Dry Weight Concentration and Culture Turbidity For A Recombinant E. Coli K12 Strain Producing High Levels of Human Alpha Interferon Analogue
Author(s) -
Fieschko John,
Ritch Tom,
Bengston David,
Fenton Dennis,
Mann Michael
Publication year - 1985
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.5420010310
Subject(s) - recombinant dna , strain (injury) , plasmid , microbiology and biotechnology , interferon , turbidity , dry weight , biology , cell culture , escherichia coli , chemistry , gene , virology , biochemistry , genetics , botany , anatomy , ecology
A recombinant E. Coli K12 strain carrying a runaway‐type plasmid with the gene for human alpha interferon analogue and capable of producing high levels of recombinant product [1], was grown in rich medium and compared to the host strain without plasmid, and to the strain with the plasmid, but lacking the interferon gene insert. At a non‐inducing temperature, gross cell morphology and the correlation between dry weight and culture turbidity was comparable for the three cultures. Following induction by temperature elevation, both plasmid‐bearing strains displayed significant cell size change, but only the interferon producing strain underwent a decrease in the ratio of cell concentration measured by dry weight to culture turbidity.