Efficient production of D ‐glucosaminic acid from D ‐glucosamine by Pseudomonas putida GNA5

D ‐glucosaminic acid was produced efficiently from glucosamine by oxidative fermentation using a newly isolated strain, Pseudomonas putida GNA5. After optimization of the fermentation process, 51.5 g L −1 D ‐glucosaminic acid was produced from an initial concentration of 60 g L −1 D ‐glucosamine‐HCl after 72 h of oxidative fermentation, which corresponded to a molar yield of 95.4%. This production process is potentially of considerable economic significance because very few by‐products were detected. Furthermore, D ‐glucosaminic acid was accumulated stably during the oxidative fermentation process without the addition of an inhibitor of D ‐glucosaminic acid breakdown, even though D ‐glucosamine was exhausted. These results suggest that the mechanisms of D ‐glucosaminic acid‐related metabolism differ between Pseudomonas putida GNA5 and the strain Pseudomonas genera , which was previously reported to produce D ‐glucosaminic acid. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011