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Efficient production of D ‐glucosaminic acid from D ‐glucosamine by Pseudomonas putida GNA5
Author(s) -
Wu Bin,
Bai Zhongzhong,
Meng Xiao,
He Bingfang
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.529
Subject(s) - pseudomonas putida , fermentation , chemistry , pseudomonas , yield (engineering) , biochemistry , oxidative phosphorylation , glucosamine , strain (injury) , metabolism , food science , bacteria , biology , enzyme , materials science , anatomy , metallurgy , genetics
D ‐glucosaminic acid was produced efficiently from glucosamine by oxidative fermentation using a newly isolated strain, Pseudomonas putida GNA5. After optimization of the fermentation process, 51.5 g L −1 D ‐glucosaminic acid was produced from an initial concentration of 60 g L −1 D ‐glucosamine‐HCl after 72 h of oxidative fermentation, which corresponded to a molar yield of 95.4%. This production process is potentially of considerable economic significance because very few by‐products were detected. Furthermore, D ‐glucosaminic acid was accumulated stably during the oxidative fermentation process without the addition of an inhibitor of D ‐glucosaminic acid breakdown, even though D ‐glucosamine was exhausted. These results suggest that the mechanisms of D ‐glucosaminic acid‐related metabolism differ between Pseudomonas putida GNA5 and the strain Pseudomonas genera , which was previously reported to produce D ‐glucosaminic acid. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011