Premium
Improved particle counting and size distribution determination of aggregated virus populations by asymmetric flow field‐flow fractionation and multiangle light scattering techniques
Author(s) -
McEvoy Matt,
Razinkov Vladimir,
Wei Ziping,
CasasFinet Jose R.,
Tous Guillermo I.,
Schenerman Mark A.
Publication year - 2011
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.499
Subject(s) - multiangle light scattering , fractionation , field flow fractionation , particle size distribution , particle (ecology) , particle size , light scattering , polystyrene , dynamic light scattering , scattering , flow (mathematics) , analytical chemistry (journal) , materials science , chemistry , chromatography , biological system , optics , nanotechnology , nanoparticle , physics , polymer , mechanics , composite material , geology , biology , oceanography
A method using a combination of asymmetric flow field‐flow fractionation (AFFFF) and multiangle light scattering (MALS) techniques has been shown to improve the estimation of virus particle counts and the amount of aggregated virus in laboratory samples. The method is based on the spherical particle counting approach given by Wyatt and Weida in 2004, with additional modifications. The new method was tested by analyzing polystyrene beads and adenovirus samples, both having a well‐characterized particle size and concentration. Influenza virus samples were analyzed by the new AFFFF‐MALS technique, and particle size and aggregate state were compared with results from atomic force microscopy analysis. The limitations and source of possible errors for the new AFFFF‐MALS analysis are discussed. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010