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Human hematopoietic progenitor cells grow faster under rotational laminar flows
Author(s) -
Murugappan G.,
CarrilloCocom L. M.,
Johnson K. E.,
GonzálezBarrón M. T.,
MorenoCuevas J. E.,
Alvarez M. M.
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.440
Subject(s) - progenitor cell , laminar flow , cd34 , haematopoiesis , progenitor , chemistry , exponential growth , biology , microbiology and biotechnology , stem cell , physics , mechanics , quantum mechanics
We report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54–58.5%, and (c) reduced doubling time in their exponential phase by 12–16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010