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Monitoring oxygen uptake in 3D tissue engineering scaffolds by phosphorescence quenching microscopy
Author(s) -
Guaccio Angela,
Netti Paolo A.
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.438
Subject(s) - quenching (fluorescence) , phosphorescence , oxygen , microscopy , computer science , polymer , biological system , work (physics) , chemistry , materials science , biomedical engineering , nanotechnology , biochemical engineering , biology , organic chemistry , optics , mechanical engineering , fluorescence , physics , engineering
Measuring oxygen concentration in three‐dimensional cultures, without interfering with cellular activities, is a fundamental request of tissue engineering research. Among the other techniques, it has been demonstrated that phosphorescence quenching microscopy (PQM) represents a valid tool for the detection of oxygen concentration in 3D environments. Indeed, it is not invasive, with high spatial and temporal resolution, and, once calibrated, it is not affected by the presence of extracellular matrix components and other environmental factors. In this work, a description of the PQM experimental set up for oxygen measurements in solutions and 3D polymer‐based cellular constructs is provided. Moreover, the advantage and the limits in the use of this technique are critically discussed to provide a technical note for future applications. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010