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Chromosomal integration of a synthetic expression control sequence achieves poly‐γ‐glutamate production in a Bacillus subtilis strain
Author(s) -
Yeh ChuanMei,
Wang JyhPerng,
Lo ShihChing,
Chan WenChia,
Lin MingYi
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.417
Subject(s) - bacillus subtilis , mutant , strain (injury) , metabolic engineering , gene , ammonium chloride , chemistry , biology , biochemistry , genetics , bacteria , organic chemistry , anatomy
Poly‐γ‐glutamate (γ‐PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the γ‐PGA synthesis ywsC‐ywtAB genes in its chromosome, cannot produce γ‐PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in γ‐PGA‐producing B. subtilis mutant strains. Mutant B. subtilis PGA6‐2 stably produces high levels of γ‐PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6‐2 is not only a γ ‐PGA producer, but it is also a candidate for the genetic and metabolic engineering of γ ‐PGA production. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010