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Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food
Author(s) -
Tippkötter Nils,
Roikaew Wipa,
Ulber Roland,
Hoffmann Alexander,
Denzler HansJörg,
Buchholz Heinrich
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.384
Subject(s) - paracoccus denitrificans , denitrification , nitrate , chemistry , effluent , nitrite , denitrifying bacteria , hydraulic retention time , filtration (mathematics) , polyvinyl alcohol , pilot plant , environmental chemistry , pulp and paper industry , nitrogen , chromatography , environmental engineering , environmental science , biochemistry , organic chemistry , enzyme , engineering , statistics , mathematics
Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource‐conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol‐immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L −1 and crossflow units with membrane areas from 0.02 to 0.80 m 2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed. © 2010 American Institute of Chemical Engineers Biotechnol. Prog. 2010