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Strategies to enhance cell growth and achieve high‐level oil production of a Chlorella vulgaris isolate
Author(s) -
Chen ChunYen,
Yeh KueiLing,
Su HueiMeei,
Lo YungChung,
Chen WenMing,
Chang JoShu
Publication year - 2010
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.381
Subject(s) - chlorella vulgaris , biomass (ecology) , photobioreactor , biodiesel , saturation (graph theory) , growth rate , nitrogen , chemistry , autotroph , food science , botany , algae , zoology , pulp and paper industry , biology , biochemistry , ecology , bacteria , organic chemistry , mathematics , geometry , genetics , combinatorics , engineering , catalysis
The autotrophic growth of an oil‐rich indigenous microalgal isolate, identified as Chlorella vulgaris CC, was promoted by using engineering strategies to obtain the microalgal oil for biodiesel synthesis. Illumination with a light/dark cycle of 14/10 (i.e., 14 h light‐on and 10 h light‐off) resulted in a high overall oil production rate ( v oil ) of 9.78 mg/L/day and a high electricity conversion efficiency ( E c ) of 23.7 mg cell/kw h. When using a NaHCO 3 concentration of 1,500 mg/L as carbon source, the v oil and E c were maximal at 100 mg/L/day and 128 mg/kw h, respectively. A Monod type model was used to describe the microalgal growth kinetics with an estimated maximum specific growth rate ( μ max ) of 0.605 day −1 and a half saturation coefficient ( K s ) of 124.9 mg/L. An optimal nitrogen source (KNO 3 ) concentration of 625 mg/L could further enhance the microalgal biomass and oil production, leading to a nearly 6.19 fold increase in v oil value. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010