Premium
Multicomponent cellulase production by Cellulomonas biazotea NCIM‐2550 and its applications for cellulosic biohydrogen production
Author(s) -
Saratale Ganesh D.,
Saratale Rijuta G.,
Lo YungChung,
Chang JoShu
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.342
Subject(s) - cellulase , bagasse , xylan , chemistry , hydrolysate , food science , cellulosic ethanol , cellulose , xylanase , reducing sugar , carboxymethyl cellulose , biohydrogen , sugar , hydrolysis , hemicellulose , clostridium butyricum , amylase , biochemistry , microbiology and biotechnology , fermentation , enzyme , organic chemistry , biology , sodium , hydrogen production , catalysis
Among four cellulolytic microorganisms examined, Cellulomonas biazotea NCIM‐2550 can grow on various cellulosic substrates and produce reducing sugar. The activity of cellulases (endoglucanase, exoglucanase, and cellobiase), xylanase, amylase, and lignin class of enzymes produced by C . biazotea was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (carboxymethyl cellulose [CMC], sugarcane bagasse [SCB], and xylan) used for growth. Effects of physicochemical conditions on cellulolytic enzyme production were systematically investigated. Using MnCl 2 as a metal additive significantly induces the cellulase enzyme system, resulting in more reducing sugar production. The efficiency of fermentative conversion of the hydrolyzed SCB and xylan into clean H 2 energy was examined with seven H 2 ‐producing pure bacterial isolates. Only Clostridium butyricum CGS5 exhibited efficient H 2 production performance with the hydrolysate of SCB and xylan. The cumulative H 2 production and H 2 yield from using bagasse hydrolysate (initial reducing sugar concentration = 1.545 g/L) were approximately 72.61 mL/L and 2.13 mmol H 2 /g reducing sugar (or 1.91 mmol H 2 /g cellulose), respectively. Using xylan hydrolysate (initial reducing sugar concentration = 0.345 g/L) as substrate could also attain a cumulative H 2 production and H 2 yield of 87.02 mL/L and 5.03 mmol H 2 /g reducing sugar (or 4.01 mmol H 2 /g cellulose), respectively. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010