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A multiparallel bioreactor for the cultivation of mammalian cells in a 3D‐ceramic matrix
Author(s) -
Goralczyk Vicky,
Driemel Gregor,
Bischof Andreas,
Peter Andrea,
Berthold Almuth,
Kroh Lothar,
Blessing Lucienne,
Schubert Helmut,
King Rudibert
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.335
Subject(s) - bioreactor , matrix (chemical analysis) , process engineering , porosity , modular design , volume (thermodynamics) , ceramic , biochemical engineering , pulp and paper industry , materials science , computer science , environmental science , chemical engineering , chemistry , biological system , biology , chromatography , composite material , engineering , physics , organic chemistry , quantum mechanics , operating system
Abstract For adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface‐to‐volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set‐up the system together with handling procedures for long‐time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices' caverns. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010