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Effect of particle size distribution on filtration performance of cell culture medium
Author(s) -
Chan Karen,
Tomioka Yusuke,
Mok Yanglin,
ITO Takao
Publication year - 2021
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.3130
Subject(s) - filtration (mathematics) , particle size , particle size distribution , particle (ecology) , filter (signal processing) , biological system , chromatography , cell size , fouling , materials science , process engineering , biochemical engineering , chemistry , chemical engineering , biology , mathematics , computer science , statistics , microbiology and biotechnology , membrane , engineering , biochemistry , ecology , computer vision
Cell culture media used in CHO‐based biologic processes are typically sterile filtered to prevent microbial contamination prior to inoculation. In this study, the impact of common sterile filter throughput on a different, commercially available cell culture media was evaluated from the intermediate‐adsorption fouling model of the filtration model. The key particle size range for optimum filter performance was discussed and identified by measuring the submicron order particle size distribution. It may be possible to predict the performance of filter capacity with size‐exclusive separation by understanding the media particle counts and size distribution.