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Anti‐ epithelial cell adhesion molecule RNA aptamer‐conjugated liposomal doxorubicin as an efficient targeted therapy in mice bearing colon carcinoma tumor model
Author(s) -
Mashreghi Mohammad,
Zamani Parvin,
Karimi Maryam,
Mehrabian Amin,
Arabsalmani Mahdieh,
Zarqi Javad,
Moosavian Seyedeh Alia,
Jaafari Mahmoud Reza
Publication year - 2021
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.3116
Subject(s) - epithelial cell adhesion molecule , cd44 , aptamer , doxorubicin , biodistribution , chemistry , in vivo , cancer research , liposome , targeted therapy , cell culture , cell , microbiology and biotechnology , pharmacology , cancer , in vitro , biology , biochemistry , medicine , chemotherapy , genetics
To overcome the lack of selectivity and nonspecific biodistribution of drugs in the body, targeted delivery of chemotherapeutic agents with aptamers is a very effective method. In this strategy, aptamers could be specifically identified and attach to targeted molecules on the cancerous cells and deliver the chemotherapeutic agents to desired tissue with minimal or no damage to the normal cells. In this study, we designed anti‐epithelial cell adhesion molecule (EpCAM) RNA aptamer conjugated PEGylated liposomal doxorubicin (ER‐lip) to investigate its in vitro and in vivo anticancer abilities. Data showed that EpCAM aptamer was able to enhance cell uptake and cytotoxic effects of Dox in C26 cell line. The biodistribution study indicated that ER‐lip enhanced the tumor accumulation of Dox compared to Caelyx. Also, double staining of isolated tumor cells with anti‐CD44‐PE‐cy5 and anti‐EpCAM Cy‐7 antibodies indicated that tumor cells expressed a high level of EpCAM + CD44 + cells ( p ≤ .001) compared to cultured C26 cell line. in vivo results showed that ER‐lip promoted survival and reduced tumor growth rate in animal model compared to Caelyx. In conclusion, these results suggested that the ER‐lip could be served as promising formulation for the treatment of cancers with the high expression of EpCAM.