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Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum
Author(s) -
Maqbool Asma,
Abbas Waseem,
Rao Abdul Qayyum,
Irfan Muhammad,
Zahur Muzna,
Bakhsh Allah,
Riazuddin Shiekh,
Husnain Tayyab
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.306
Subject(s) - cauliflower mosaic virus , gossypium , biology , transgene , gene , genetically modified crops , complementary dna , heat shock protein , gossypium hirsutum , drought stress , genomic dna , drought tolerance , botany , genetics
Heat‐shock proteins (HSP) are molecular chaperones for protein molecules. These proteins play an important role in protein–protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. A small HSP gene GHSP26 present in Gossypium arboreum responds to dehydration. In the present study, an attempt was made to overcome the problem of drought stress in cotton. A cDNA of GHSP26 was isolated from G. arboreum , cloned in plant expression vector, pCAMBIA‐1301 driven by the cauliflower mosaic virus 35S promoter and introduced into Gossypium hirsutum . The integration and expression studies of putative transgenic plants were performed through GUS assay; PCR from genomic DNA, and quantitative real‐time PCR analysis. Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010