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Inverse liquid‐solid chromatography to evaluate drug interactions with organosilane‐modified polydimethylsiloxane for use in body‐on‐a‐chip systems
Author(s) -
Schnepper Mark,
Roles Jeff,
Hickman James J.
Publication year - 2020
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.3048
Subject(s) - polydimethylsiloxane , adsorption , chromatography , inverse gas chromatography , materials science , polyethylene glycol , chemistry , chemical engineering , nanotechnology , organic chemistry , engineering
Body‐on‐a‐chip and organ‐on‐a‐chip systems utilize polydimethylsiloxane (PDMS) because of the relative suitability of the material for fabrication of microfluidic channels and chambers used in these devices. However, hydrophobic molecules, especially therapeutic compounds, tend to adsorb to PDMS, which may distort the dose–response curves that feed into the pharmacokinetic/pharmacodynamic models used to translate preclinical data into predictions of clinical outcomes. Surface modification by organosilanes is one method being explored to modify PDMS, but the effect of organosilanes on drug adsorption isotherms is not well characterized. We utilized Inverse Liquid‐Solid Chromatography to characterize the adsorption parameters of the drugs acetaminophen, diclofenac, and verapamil with native PDMS and organosilane‐modified (fluoropolymer (13F) and polyethylene glycol) PDMS surfaces, to correlate the modifications with changes in drug adsorption. It was determined that the organosilane modifications significantly changed the energy of adsorption of the test drug utilizing our methodology.