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Biotransformation of cladribine using a stabilized biocatalyst in calcium alginate beads
Author(s) -
Lapponi María J.,
Britos Claudia N.,
Rivero Cintia W.,
Trelles Jorge A.
Publication year - 2019
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2927
Subject(s) - cladribine , bioprocess , biotransformation , bioconversion , biocatalysis , chemistry , calcium alginate , nucleoside , chromatography , biochemistry , catalysis , organic chemistry , calcium , fermentation , biology , enzyme , medicine , reaction mechanism , paleontology
Cladribine is a nucleoside analogue widely used in the pharmaceutical industry for the treatment of several neoplasms, including hairy‐cell leukemia among others. This compound has also shown efficacy in the treatment of autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. In this work, a green bioprocess for cladribine biosynthesis using immobilized Arthrobacter oxydans was developed. The microorganism was stabilized by entrapment immobilization in the natural matrix alginate. Different reaction parameters were optimized obtaining a biocatalyst able to achieve cladribine bioconversion values close to 85% after 1 hr, the shortest reaction times reported so far. The developed bioprocess was successfully scaled‐up reaching a productivity of 138 mg L −1 hr −1 . Also, the biocatalyst was stable for 5 months in storage and in 96 hr at operational conditions.