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Fed‐batch production of tetanus toxin by Clostridium tetani
Author(s) -
Fratelli Fernando,
Siquini Tatiana Joly,
de Abreu Marcelo Estima,
Higashi Hisako Gondo,
Converti Attilio,
de Carvalho João Carlos Monteiro
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.292
Subject(s) - clostridium tetani , toxin , tetanus , chemistry , yield (engineering) , microbiology and biotechnology , food science , clostridium , biology , biochemistry , bacteria , materials science , vaccination , genetics , metallurgy , immunology
Abstract This study deals with the effects of the initial nitrogen source (NZ Case TT) level and the protocol of glucose addition during the fed‐batch production of tetanus toxin by Clostridium tetani . An increase in the initial concentration of NZ Case TT (NZ 0 ) accelerated cell growth, increased the consumption of the nitrogen source as well as the final yield of tetanus toxin, which achieved the highest values (50–60 L f /mL) for NZ 0 ≥ 50 g/L. The addition of glucose at fixed times (16, 56, and 88 h) ensured a toxin yield (∼60 L f /mL) about 33% higher than those of fed‐batch runs with addition at fixed concentration (∼45 L f /mL) and about 300% higher than those obtained in reference batch runs nowadays used at industrial scale. The results of this work promise to substantially improve the present production of tetanus toxin and may be adopted for human vaccine production after detoxification and purification. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010