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Factors affecting sedimentational separation of bacteria from blood
Author(s) -
Pitt William G.,
Alizadeh Mahsa,
Blanco Rae,
Hunter Alex K.,
Bledsoe Colin G.,
McClellan Daniel S.,
Wood Madison E.,
Wood Ryan L.,
Ravsten Tanner V.,
Hickey Caroline L.,
Cameron Beard William,
Stepan Jacob R.,
Carter Alexandra,
Husseini Ghaleb A.,
Robison Richard A.,
Welling Evelyn,
Torgesen Rebekah N.,
Anderson Clifton M.
Publication year - 2019
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2892
Subject(s) - sedimentation , bacteria , spinning , plasma , chromatography , volume (thermodynamics) , separation (statistics) , chemistry , materials science , analytical chemistry (journal) , biology , physics , composite material , mathematics , thermodynamics , statistics , paleontology , genetics , quantum mechanics , sediment
Abstract Rapid diagnosis of blood infections requires fast and efficient separation of bacteria from blood. We have developed spinning hollow disks that separate bacteria from blood cells via the differences in sedimentation velocities of these particles. Factors affecting separation included the spinning speed and duration, and disk size. These factors were varied in dozens of experiments for which the volume of separated plasma, and the concentration of bacteria and red blood cells (RBCs) in separated plasma were measured. Data were correlated by a parameter of characteristic sedimentation length, which is the distance that an idealized RBC would travel during the entire spin. Results show that characteristic sedimentation length of 20 to 25 mm produces an optimal separation and collection of bacteria in plasma. This corresponds to spinning a 12‐cm‐diameter disk at 3,000 rpm for 13 s. Following the spin, a careful deceleration preserves the separation of cells from plasma and provides a bacterial recovery of about 61 ± 5%.