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Impact of the type of microcarrier and agitation modes on the expansion performances of mesenchymal stem cells derived from umbilical cord
Author(s) -
Loubière Céline,
Sion Caroline,
De Isla Natalia,
Reppel Loic,
Guedon Emmanuel,
Chevalot Isabelle,
Olmos Eric
Publication year - 2019
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2887
Subject(s) - microcarrier , mesenchymal stem cell , umbilical cord , biomedical engineering , stem cell , chemistry , materials science , andrology , microbiology and biotechnology , cell , biology , immunology , medicine , biochemistry
The present study proposed to compare the impact of agitation mode (static, orbital, and mechanical) on the culture of mesenchymal stem cells extracted from the Wharton's jelly of umbilical cords (WJ‐MSC), in a clinical grade culture medium, using human platelet lysate and different xeno‐free microcarriers. Attachment, expansion, and detachment performances were characterized by a new dedicated tool of microscopic image posttreatment, allowing an in situ cell counting without detachment step. Results showed that performances in static mode were not necessarily representative of those obtained in dynamic mode. Moreover, impacts on nutrient consumptions and metabolite productions were identified, such as a higher glutamine consumption when Cytodex‐1 microcarriers were used. The detachment strategy used was relatively efficient for Star‐Plus, Plastic‐Plus, and Hillex II, but not sufficient for Cytodex‐1. Despite Cytodex‐1 presented promising attachment and expansion performances, Star‐Plus and Plastic‐Plus showed a better compromise, respectively, for the orbital and the mechanical agitation modes.