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Parallel gene cloning and protein production in multiple expression systems
Author(s) -
Wang HuiMin,
Shih YanPing,
Hu SuMing,
Lo WenTsung,
Lin HuiMin,
Ding ShihShiu,
Liao HsinChi,
Liang PoHuang
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.274
Subject(s) - xhoi , cloning (programming) , recombinant dna , ecori , gene , biology , molecular cloning , restriction site , computational biology , cloning vector , genetics , restriction enzyme , vector (molecular biology) , microbiology and biotechnology , gene expression , computer science , programming language
Abstract To quickly find an optimal expression system for recombinant protein production, a set of vectors with the same restriction sites were constructed for parallel cloning of a target gene and recombinant protein production in prokaryotic and eukaryotic expression systems, simultaneously. These vectors include nucleotide sequences encoding protein tags and protease recognition sites for tag removal, followed by the cloning sites 5′‐EcoRI/3′‐XhoI identical in these vectors for ligating with the sticky‐end PCR product of a target gene. Our vectors allow parallel gene cloning and protein production in multiple expression systems with minimal cloning effort. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009