Capturing B type acute lymphoblastic leukemia cells using two types of antibodies
Author(s) -
İçöz Kutay,
Gerçek Tayyibe,
Murat Ayşegül,
Özcan Servet,
Ünal Ekrem
Publication year - 2018
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2737
Subject(s) - biochip , flow cytometry , antibody , lymphoblastic leukemia , immunomagnetic separation , microfluidics , chromatography , minimal residual disease , materials science , chemistry , microbiology and biotechnology , biomedical engineering , immunology , nanotechnology , leukemia , medicine , biology
One way to monitor minimal residual disease (MRD) is to screen cells for multiple surface markers using flow cytometry. In order to develop an alternative microfluidic based method, isolation of B type acute lymphoblastic cells using two types of antibodies should be investigated. The immunomagnetic beads coated with various antibodies are used to capture the B type acute lymphoblastic cells. Single beads, two types of beads and surface immobilized antibody were used to measure the capture efficiency. Both micro and nanosize immunomagnetic beads can be used to capture B type acute lymphoblastic cells with a minimum efficiency of 94% and maximum efficiency of 98%. Development of a microfluidic based biochip incorporating immunomagnetic beads and surface immobilized antibodies for monitoring MRD can be an alternative to current cost and time inefficient laboratory methods. © 2018 American Institute of Chemical Engineers Biotechnol. Prog ., 35: e2737, 2019
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