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Rapid creation system of morphologically and functionally communicative three‐dimensional cell‐dense tissue by centrifugation
Author(s) -
Haraguchi Yuji,
Matsuura Katsuhisa,
Kagawa Yuki,
Hasegawa Akiyuki,
Kubo Hirotsugu,
Shimizu Tatsuya
Publication year - 2018
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2691
Subject(s) - centrifugation , c2c12 , myocyte , cell , microbiology and biotechnology , tissue culture , incubation , differential centrifugation , tissue engineering , cell culture , induced pluripotent stem cell , biology , chemistry , embryonic stem cell , biophysics , anatomy , biochemistry , in vitro , genetics , myogenesis , gene
This study reports a rapid fabrication system of a morphologically and functionally communicative three‐dimensional (3D) cell‐dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g , 37 °C, 30 min). A thicker tissue created on a temperature‐responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 μm‐thickness were created at 1.5 h [centrifugation (80 x g , 37 °C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell‐derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37 °C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. © 2018 American Institute of Chemical Engineers Biotechnol. Prog ., 34:1447–1453, 2018