Premium
Combined effect of attrition and ultrasound on the disruption of Pseudomonas putida for the efficient release of arginine deiminase
Author(s) -
Patil Mahesh D.,
Shinde Ashok S.,
Dev Manoj J.,
Patel Gopal,
Bhilare Kiran D.,
Banerjee Uttam Chand
Publication year - 2018
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2664
Subject(s) - sonication , pseudomonas putida , chemistry , slurry , arginine deiminase , chromatography , kinetics , arginine , nuclear chemistry , materials science , biochemistry , enzyme , composite material , amino acid , physics , quantum mechanics
Disruption of Pseudomonas putida KT2440 by ultrasound treatment in a bath sonicator, in presence of the glass beads, was carried out for the release of arginine deiminase (ADI) and the results were compared with that of by Dyno‐mill. The release of ADI depended mainly on the bead size and cellmass concentration being disrupted in bead mill. Nearly 23 U mL −1 ADI was released when slurry with a cell‐mass concentration of 250 g L −1 was disintegrated for 9 min with 80% bead loading (0.25 mm) in Dyno‐mill. Marginally higher amount of ADI (24.1 U mL −1 ) was released by the bath sonication of 250 g L −1 cellmass slurry for 30 min with the beads (0.1 mm) and a sonication power of 170 W. The glass beads, suspended along with the cellmass slurry in bath sonicator, efficiently disrupted the microbial cells to release ADI. Variation in the kinetic constants for the performance parameters implied that ADI release and cell disruption kinetics is a function of disruption technique used and the process variables thereof. Estimation of location factor suggested that selective release of ADI can be achieved. © 2018 American Institute of Chemical Engineers Biotechnol. Prog. , 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog. , 34:1185–1194, 2018