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Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white
Author(s) -
Hirsch Daniela B.,
Baieli María F.,
Urtasun Nicolás,
Lázaro Martínez Juan M.,
Glisoni Romina J.,
Miranda María V.,
Cascone Osvaldo,
Wolman Federico J.
Publication year - 2017
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2588
Subject(s) - sulfanilic acid , lysozyme , chitosan , egg white , chemistry , white (mutation) , chromatography , biochemistry , organic chemistry , gene
A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico‐chemically characterized by ss‐NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g −1 while the dissociation constant was 0.074 ± 0.012 mg mL −1 . The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP‐HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog. , 34:387–396, 2018