Premium
Assembly of breast cancer heterotypic spheroids on hyaluronic acid coated surfaces
Author(s) -
Carvalho Marco P.,
Costa Elisabete C.,
Correia Ilídio J.
Publication year - 2017
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2497
Subject(s) - spheroid , hyaluronic acid , extracellular matrix , agarose , adhesion , cancer cell , cell adhesion , chemistry , cell , 3d cell culture , cancer , biomedical engineering , biophysics , microbiology and biotechnology , nanotechnology , biology , materials science , in vitro , biochemistry , anatomy , medicine , genetics , organic chemistry
Drug screening is currently demanding for realistic models that are able to reproduce the structural features of solid tumors. 3D cell culture systems, namely spheroids, emerged as a promising approach to provide reliable results during drug development. So far, liquid overlay technique (LOT) is one of the most used methods for spheroids assembly. It comprises cellular aggregation due to their limited adhesion to certain biomaterials, like agarose. However, researchers are currently improving this technique in order to obtain spheroids on surfaces that mimic cancer extracellular matrix (ECM), since cell–ECM interactions modulate cells behavior and their drug resistance profile. Herein, hyaluronic acid (HA) coated surfaces were used, for the first time, for the production of reproducible heterotypic breast cancer spheroids. The obtained results revealed that it is possible to control the size, shape, and number of spheroids gotten per well by changing the HA concentration and the number of cells initially seeded in each well. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1346–1357, 2017