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Poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) production from biodiesel by‐product and propionic acid by mutant strains of Pandoraea sp.
Author(s) -
de Paula Fabrício C.,
de Paula Carolina B. C.,
Gomez José Gregório C.,
Steinbüchel Alexander,
Contiero Jonas
Publication year - 2017
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2481
Subject(s) - glycerol , chemistry , yield (engineering) , mutant , propionate , biodiesel , strain (injury) , valeric acid , polyhydroxyalkanoates , biodiesel production , nuclear chemistry , food science , biochemistry , organic chemistry , bacteria , catalysis , biology , materials science , acetic acid , genetics , anatomy , metallurgy , gene
Pandoraea sp. MA03 wild type strain was subjected to UV mutation to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) [P(3HB‐ co ‐3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 g g −1 . The use of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21.9 to 30 mol% and 22.2 to 36.7 mol%, respectively. Fed‐batch fermentations were performed using CG and PA and reached a 3HV yield of 1.16 g g −1 , which is 86% of the maximum theoretical yield. Nitrogen limitation was a key parameter for polymer accumulation reaching up to 63.7% content and 18.1 mol% of 3HV. Henceforth, mutant prp25 is revealed as an additional alternative to minimize costs and support the P(3HB‐ co ‐3HV) production from biodiesel by‐products. © 2017 American Institute of Chemical Engineers Biotechnol. Prog. , 33:1077–1084, 2017