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Optimization‐based framework for resin selection strategies in biopharmaceutical purification process development
Author(s) -
Liu Songsong,
Gerontas Spyridon,
Gruber David,
Turner Richard,
TitchenerHooker Nigel J.,
Papageorgiou Lazaros G.
Publication year - 2017
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2479
Subject(s) - biopharmaceutical , selection (genetic algorithm) , process (computing) , yield (engineering) , microscale chemistry , computer science , integer programming , flexibility (engineering) , best linear unbiased prediction , process development , mathematical optimization , integer (computer science) , nonlinear programming , biochemical engineering , nonlinear system , process engineering , algorithm , engineering , mathematics , microbiology and biotechnology , materials science , machine learning , statistics , mathematics education , physics , quantum mechanics , metallurgy , biology , programming language , operating system
This work addresses rapid resin selection for integrated chromatographic separations when conducted as part of a high‐throughput screening exercise during the early stages of purification process development. An optimization‐based decision support framework is proposed to process the data generated from microscale experiments to identify the best resins to maximize key performance metrics for a biopharmaceutical manufacturing process, such as yield and purity. A multiobjective mixed integer nonlinear programming model is developed and solved using the ε‐constraint method. Dinkelbach's algorithm is used to solve the resulting mixed integer linear fractional programming model. The proposed framework is successfully applied to an industrial case study of a process to purify recombinant Fc Fusion protein from low molecular weight and high molecular weight product related impurities, involving two chromatographic steps with eight and three candidate resins for each step, respectively. The computational results show the advantage of the proposed framework in terms of computational efficiency and flexibility. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog. , 33:1116–1126, 2017