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E nrichment of apoplastic fluid with therapeutic recombinant protein for efficient biofarming
Author(s) -
Chatterjee Aparajita,
Das Narayan C.,
Raha Sumita,
Maiti Indu B.,
Shrestha Ankita,
Khan Ahamed,
Acharya Sefali,
Dey Nrisingha
Publication year - 2017
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2461
Subject(s) - nicotiana tabacum , nicotiana benthamiana , recombinant dna , transgene , agrobacterium , genetically modified crops , nicotiana , biopharmaceutical , transformation (genetics) , biology , botany , microbiology and biotechnology , solanaceae , gene , biochemistry
Objective For efficient biofarming we attempted to enrich plant interstitial fluid (IF)/apoplastic fluid with targeted recombinant therapeutic protein. We employed a synthetic human Glucocerebrosidase (GCB), a model biopharmaceutical protein gene in this study. Results Twenty one Nicotiana varieties, species and hybrids were initially screened for individual IF recovery and based on the findings, we selected Nicotiana tabacum NN (S‐9‐6), Nicotiana tabacum nn (S‐9‐7) and Nicotiana benthamiana (S‐6‐6) as model plants for raising transgenic expressing GCB via Agrobacterium mediated transformation under the control of M24 promoter; GCB specific activity in each transgenic lines were analyzed and we observed higher concentration of recombinant GCB in IF of these transgenic lines (S‐9‐6, S‐9‐7, and S‐6‐6) in comparison to their concentration in crude leaf extracts. Conclusion Recovery of valuable therapeutics in plant IF as shown in the present study holds great promise for promoting plant based biofarming. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:726–736, 2017