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Opossum peptide that can neutralize rattlesnake venom is expressed in E scherichia coli
Author(s) -
Komives Claire F.,
Sanchez Elda E.,
Rathore Anurag S.,
White Brandon,
Balderrama Michael,
Suntravat Montamas,
Cifelli Angela,
Joshi Varsha
Publication year - 2016
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2386
Subject(s) - venom , peptide , crotalus , antivenom , recombinant dna , protease , envenomation , chemistry , myotoxin , microbiology and biotechnology , biology , pharmacology , biochemistry , snake venom , enzyme , gene
An eleven amino acid ribosomal peptide was shown to completely neutralize Western Diamondback Rattlesnake (Crotalus atrox) venom in mice when a lethal dose of the venom was pre‐incubated with the peptide prior to intravenous injection. We have expressed the peptide as a concatenated chain of peptides and cleaved them apart from an immobilized metal affinity column using a protease. After ultrafiltration steps, the mixture was shown to partially neutralize rattlesnake venom in mice. Preliminary experiments are described here that suggest a potential life‐saving therapy could be developed. To date, no recombinant therapies targeting cytotoxic envenomation have been reported. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:81–86, 2017