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Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies
Author(s) -
Méndez Arias Johanna,
Modesto Luiz Felipe Amarante,
Polikarpov Igor,
Pereira Nei
Publication year - 2016
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2306
Subject(s) - bagasse , bioconversion , hemicellulose , response surface methodology , hydrolysis , cellulose , central composite design , chemistry , enzymatic hydrolysis , lignin , aspergillus niger , trichoderma harzianum , food science , yield (engineering) , biomass (ecology) , lignocellulosic biomass , cellulosic ethanol , microbiology and biotechnology , biochemistry , chromatography , botany , fermentation , materials science , organic chemistry , biology , agronomy , metallurgy , biological pest control
Lignocellulosic materials represent a very important and promising source of renewable biomass. In order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. Experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of Trichoderma harzianum IOC 3844, Penicillium funiculosum ATCC 11797, and Aspergillus niger ATCC 1004, resulting in a proportion of 15, 50, and 35%, respectively. This mixture was able to hydrolyze 25 g/L of pretreated sugarcane bagasse with 91% of yield after 48 h of enzymatic reaction. Synergism along the hydrolysis process, besides the influence of lignin, hemicellulose, and solids loading, were also studied. Response surface methodology (RSM) based on Central Composite Rotatable Design was used to optimize solids and protein loadings to increase glucose release and enzymatic hydrolysis yield. The optimum solid and protein loadings established with RSM were 196 g/L and 24 mg/g cellulose, respectively, and under these conditions (94.1 ± 8) g/L of glucose were obtained, corresponding to a hydrolysis yield of 64%. © 2016 American Institute of Chemical Engineers Biotechnol. Prog ., 32:1222–1229, 2016