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Invariability of central metabolic flux distribution in Shewanella oneidensis MR‐1 under environmental or genetic perturbations
Author(s) -
Tang Yinjie J.,
Martin Hector Garcia,
Deutschbauer Adam,
Feng Xueyang,
Huang Rick,
Llora Xavier,
Arkin Adam,
Keasling Jay D.
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.227
Subject(s) - shewanella oneidensis , doubling time , flux (metallurgy) , metabolic pathway , metabolism , metabolic flux analysis , metabolite , chemistry , transposable element , shewanella , biochemistry , bacteria , biology , mutant , genetics , gene , in vitro , organic chemistry
An environmentally important bacterium with versatile respiration, Shewanella oneidensis MR‐1, displayed significantly different growth rates under three culture conditions: minimal medium (doubling time ∼3 h), salt stressed minimal medium (doubling time ∼6 h), and minimal medium with amino acid supplementation (doubling time ∼1.5 h). 13 C‐based metabolic flux analysis indicated that fluxes of central metabolic reactions remained relatively constant under the three growth conditions, which is in stark contrast to the reported significant changes in the transcript and metabolite profiles under various growth conditions. Furthermore, 10 transposon mutants of S. oneidensis MR‐1 were randomly chosen from a transposon library and their flux distributions through central metabolic pathways were revealed to be identical, even though such mutational processes altered the secondary metabolism, for example, glycine and C1 (5,10‐Me‐THF) metabolism. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009