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Stability, purification, and applications of bromelain: A review
Author(s) -
de Lencastre Novaes Letícia Celia,
Jozala Angela Faustino,
Lopes André Moreni,
de Carvalho SantosEbinuma Valéria,
Mazzola Priscila Gava,
Pessoa Junior Adalberto
Publication year - 2015
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2190
Subject(s) - bromelain , cysteine protease , protease , ultrafiltration (renal) , chemistry , proteases , chromatography , enzyme , biochemistry
Bromelain is a cysteine protease found in pineapple tissue. Because of its anti‐inflammatory and anti‐cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid–liquid extractions by aqueous two‐phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up‐to‐date compilation of the data reported on this enzyme. © 2015 American Institute of Chemical Engineers Biotechnol. Prog. , 32:5–13, 2016